Seventeen different groups joined in the complaint, claiming the Hoang Anh Gia Lai rubber company had destroyed forestry lands affecting as many as 2,000 families.
Prime Minister Hun Sen has ordered Phnom Penh authorities to grant permission to both sides of demonstrations to occupy Freedom Park in the capital.
The beating death of a Vietnamese-Cambodian earlier this month was the first incident in several years that some are calling a hate crime.
Supporters of the 17 hill tribe groups from northeastern Cambodia say they believe the complaint will meet World Bank standards for review.
The families moved a few kilometers outside of town, where they said they would encamp on an empty plot of land.
The petition, which was launched Thursday, calls for the courts to drop the charges against the detainees and release them without conditions.
Nine people were injured, three of them seriously, when housing rights demonstrators clashed with police on Friday morning.
At least four people were killed and dozens injured when police opened fire on protesters Jan. 3.
Reporters Without Borders has put Cambodia on a list of countries in a “difficult situation” in its annual Press Freedom Index.
Cambodia ranks No. 144 of 180 countries on the index, above China, Iran, Vietnam, Sudan and others.
The Cambodian Court of Appeals has denied bail to 21 detained labor protesters, setting the stage for more demonstrations on their behalf.
The 21 detainees were among 23 arrested in deadly labor protest crackdowns in January.